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Home >> Antibodies >> Acetyl-Histone H2A Antibody (Lys9)

Acetyl-Histone H2A Antibody (Lys9) [clone DEB-8] (RQ5085)

  Catalog No Formulation Size Price (USD)  
Image RQ5085 Antibody in PBS with 0.02% sodium azide and 50% glycerol 100 ug 359
Western blot testing of lysate from human HeLa cells treated with Trichostatin A, with acetyl-Histone H2A Antibody (Lys9). Predicted molecular weight ~14 kDa.
IHC staining of FFPE mouse brain tissue with acetyl-Histone H2A Antibody (Lys9). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Availability 1-2 weeks
Species Reactivity Human, Mouse
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name DEB-8
Purity Affinity purified
UniProt P04908
Localization Nuclear
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:500-1:1000
Limitations This acetyl-Histone H2A Antibody (Lys9) is available for research use only.
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Description

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. [UniProt]

Application Notes

Optimal dilution of the acetyl-Histone H2A Antibody (Lys9) should be determined by the researcher.

Immunogen

A synthetic peptide specific to human Histone H2A (surrounding acetylated lysine 9) was used as the immunogen for the Histone H2A antibody.

Storage

Store the acetyl-Histone H2A Antibody (Lys9) at -20oC.

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