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Home >> Antibodies >> Acetyl CoA Carboxylase 1 Antibody / ACACA

Acetyl CoA Carboxylase 1 Antibody / ACACA (RQ7317)

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  Catalog No Formulation Size Price (USD)  
Image RQ7317 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human U-2 OS cells with Acetyl CoA Carboxylase 1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human U-2 OS cells with Acetyl CoA Carboxylase 1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U-251 cells with Acetyl CoA Carboxylase 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Acetyl CoA Carboxylase 1 antibody.
Western blot testing of human 1) HeLa and 2) Jurkat cell lysate with Acetyl CoA Carboxylase 1 antibody. Observed molecular weight ~260 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q13085
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Acetyl CoA Carboxylase 1 antibody is available for research use only.
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Description

Acetyl-CoA carboxylase 1 also known as ACC-alpha or ACCa is an enzyme that in humans is encoded by the ACACA gene. Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.

Application Notes

Optimal dilution of the Acetyl CoA Carboxylase 1 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids D31-R2336) was used as the immunogen for the Acetyl CoA Carboxylase 1 antibody.

Storage

After reconstitution, the Acetyl CoA Carboxylase 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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