ACE2 Antibody for FACS / Angiotensin-Converting Enzyme 2 Antibody for Flow Cytometry (FY12414)

ACE2 Antibody for FACS in human HepG2 cells. Flow cytometry analysis using a rabbit polyclonal ACE2 antibody demonstrates a rightward fluorescence shift in HepG2 cells compared with isotype control and unstained samples, indicating ACE2-positive cells. Cells were fixed with 4% paraformaldehyde and permeabilized prior to staining. Blue line: ACE2-stained cells; green line: rabbit IgG isotype control; red line: unstained control. Detection was performed using DyLight 488-conjugated goat anti-rabbit IgG secondary antibody.

Western blot analysis of ACE2 using anti-ACE2 antibody. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human CCRF-CEM whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACE2 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. The expected molecular weight of ACE2 is 90-100 kDa.