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Home >> Antibodies >> ACAT1 Antibody

ACAT1 Antibody (F50511)

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  Catalog No Formulation Size Price (USD)  
Image F50511-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F50511-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of ACAT1 antibody at 1:2000 dilution. Lane 1: human SW620 lysate; 2: mouse liver lysate; Predicted molecular weight: ~45kDa.
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Western blot testing of ACAT1 antibody at 1:2000 dilution. Lane 1: human SW620 lysate; 2: mouse liver lysate; Predicted molecular weight: ~45kDa.
Western blot testing of ACAT1 antibody at 1:1000 dilution. Lane 1: human SK-BR-3 lysate; 2: human HepG2 lysate; Predicted molecular weight: ~45 kDa.
IHC analysis of FFPE human hepatocarcinoma tissue stained with ACAT1 antibody
Availability 1-3 business days
Species Reactivity Human, Mouse
Predicted Reactivity Primate
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P24752
Localization Cytoplasmic
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Limitations This ACAT1 antibody is available for research use only.
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Description

Acetyl-CoA acetyltransferase 1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

Application Notes

Titration of the ACAT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 296-329 from the human protein was used as the immunogen for this ACAT1 antibody.

Storage

Aliquot the ACAT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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