ACAA1 Antibody / 3-ketoacyl-CoA thiolase, peroxisomal Antibody (FY13596)

ACAA1 Antibody by IF in HeLa cells. Immunofluorescence staining using 3-ketoacyl-CoA thiolase, peroxisomal Antibody shows green cytoplasmic staining with small punctate structures and relatively weak nuclear signal. Nuclei are counterstained blue with DAPI. Cells underwent enzyme-mediated antigen retrieval for 15 minutes and blocking with 10% goat serum. Primary antibody was applied at 5 ug/mL overnight at 4oC, followed by a Fluoro488-conjugated secondary antibody at 1:500 for 30 minutes at 37oC. The observed staining distribution is described without assuming organelle-specific colocalization.

ACAA1 Antibody by IHC in human liver carcinoma. Immunohistochemistry of paraffin-embedded human liver carcinoma using 3-ketoacyl-CoA thiolase, peroxisomal Antibody reveals granular brown staining in tumor-cell cytoplasm. Sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. Primary antibody was applied at 2 ug/mL overnight at 4oC, followed by peroxidase-conjugated secondary antibody and DAB chromogen. Hematoxylin counterstains the nuclei blue.

ACAA1 Antibody by WB in human and rodent samples. Western blot analysis using 3-ketoacyl-CoA thiolase, peroxisomal Antibody detects a prominent immunoreactive band near 41 kDa in human HepG2 cells (lane 1), rat liver (lane 2), and mouse liver (lane 3). The calculated molecular weight is approximately 44 kDa. Reduced samples containing 30 ug protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. After blocking with 5% nonfat milk, the membrane was incubated with primary antibody at 0.5 ug/mL overnight at 4oC. Detection used an HRP-conjugated secondary antibody at 1:5000 and a chemiluminescent substrate. The band positions are described relative to the molecular weight standards in the blot.

ACAA1 Antibody by IHC in human renal carcinoma. Immunohistochemistry of paraffin-embedded human renal carcinoma using 3-ketoacyl-CoA thiolase, peroxisomal Antibody reveals brown staining distributed through tumor-cell cytoplasm. Sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. Primary antibody was applied at 2 ug/mL overnight at 4oC, followed by peroxidase-conjugated secondary antibody and DAB chromogen. Hematoxylin counterstains the nuclei blue.