ABO Antibody Blood Group Antigen B / ABO Antigen B [clone HEB-20] (V2552)

Description

ABO Antibody Blood Group Antigen B targets ABO Antigen B, a carbohydrate blood group determinant that is part of the ABO blood group system and defines the B blood group phenotype. ABO Antigen B is expressed on the surface of erythrocytes and is also present on epithelial and endothelial cells in various tissues. This antigen is generated through specific glycosylation of precursor chains, resulting in a terminal galactose residue that distinguishes Antigen B from Antigen A and H antigens within the ABO system.

Functionally, ABO Antigen B serves as a critical marker in transfusion medicine, transplantation biology, and histopathology. A short functional summary is that Blood Group Antigen B defines blood group B status and contributes to cell surface antigen diversity through regulated carbohydrate modification. Because of its stable expression on red blood cells and variable expression in tissues, ABO Antigen B antibody reagents are widely used to study blood group antigen distribution and tissue specific glycosylation patterns.

At the molecular level, ABO Antigen B is not a protein but a carbohydrate epitope displayed on glycoproteins and glycolipids. Its biosynthesis depends on the activity of glycosyltransferases encoded by the ABO gene locus. ABO Antibody Blood Group Antigen B tools are therefore valuable for investigating carbohydrate antigen expression, cell surface biology, and alterations in glycosylation associated with disease. Clone HEB-20 is designed to recognize ABO Antigen B and is commonly used in research and histological studies to support consistent detection of this blood group determinant.

From a biological and disease relevance perspective, changes in Blood Group Antigen B expression have been reported in certain malignancies and pathological conditions, where altered glycosylation may reflect changes in differentiation or cellular transformation. Expression of ABO Antigen B has been examined in colorectal cancer, gastric cancer, and other epithelial tumors. Clone HEB-20 provides a reliable reagent for detecting ABO Antigen B in studies of pathology, blood group antigen expression, and carbohydrate-based biomarkers.

Developmentally, ABO Antigen B expression is well established on erythroid cells and shows tissue specific regulation in epithelial and endothelial compartments. ABO Antibody Blood Group Antigen B reagents from NSJ Bioreagents are supplied for research use to support investigations in pathology, transfusion related research, and cell surface glycosylation.

Application Notes

Optimal dilution of the ABO antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A mixture of erythrocytes of group B and glycoprotein fraction isolated from saliva of secretors with blood group B was used as the immunogen for the ABO antibody.

Storage

Store the ABO antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).