ABHD5 Antibody / CGI-58 Antibody (R32489)

ABHD5 Antibody IHC in Human Colon Cancer Epithelium. Immunohistochemistry of FFPE human colon cancer tissue was performed with ABHD5 Antibody at 2 ug/ml following heat-induced epitope retrieval in pH 8 EDTA buffer. Brown staining is visible throughout the glandular tumor epithelium, with predominantly cytoplasmic labeling and heterogeneous staining intensity among the cells. The staining demonstrates detection with a CGI-58 Antibody in human colon cancer tissue.

ABHD5 Antibody IHC in Human Mucinous Colon Cancer. Immunohistochemistry of FFPE human colon cancer tissue was performed with ABHD5 Antibody at 2 ug/ml following heat-induced epitope retrieval in pH 8 EDTA buffer. Distinct brown staining is present in epithelial cells lining the malignant glands, with comparatively weaker staining in many mucin-containing cells and surrounding stromal areas. The heterogeneous staining highlights differences in ABHD5 expression across the tumor tissue.

ABHD5 Antibody IHC in Human Breast Cancer. Immunohistochemistry of FFPE human breast cancer tissue was performed with ABHD5 Antibody at 2 ug/ml following heat-induced epitope retrieval in pH 8 EDTA buffer. Brown staining is visible in tumor cells distributed between adipose spaces, with heterogeneous cytoplasmic labeling and comparatively low staining in the surrounding adipose tissue. The pattern supports detection with a CGI-58 Antibody in human breast cancer tissue.

ABHD5 Antibody Immunofluorescence in HeLa Cells. Human HeLa cells were stained with ABHD5 Antibody at 5 ug/ml (green) and Alpha Tubulin Antibody (red) following enzyme-mediated antigen retrieval. ABHD5 fluorescence is concentrated predominantly in the nuclear region, with heterogeneous granular staining within the nuclei and comparatively weaker cytoplasmic signal. Alpha tubulin staining delineates the cytoplasmic microtubule network.

ABHD5 Antibody Western Blot in Human, Rat and Mouse Samples. Western blot testing of human HeLa (lane 1), RT4 (lane 2), K562 (lane 3), and HEL (lane 4) cell lysates, rat testis (lane 5), and mouse testis (lane 6) tissue lysates was performed with ABHD5 Antibody at 0.5 ug/ml. A predominant band is detected at approximately 39 kDa across all six samples, consistent with the expected molecular weight of ABHD5. The signal is strongest in RT4 cells, with clear detection also present in the other human, rat, and mouse samples. The results support cross-species detection using a CGI-58 Antibody.