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Home >> Antibodies >> ABCG2 Antibody [Discontinued, view alternatives]

ABCG2 Antibody [Discontinued, view alternatives] (F44284)

  Catalog No Formulation Size Price (USD)  
Image F44284-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 299
Image
F44284-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
Confocal immunofluorescent analysis of ABCG2 antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
ABCG2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue
ABCG2 antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control (left histogram)
Western blot analysis of ABCG2 antibody and 293 lysate. Predicted molecular weight ~72kDa.
Availability Discontinued
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt Q9UNQ0
Applications Western blot : 1:1000
Immunofluorescence : 1:10-1:50
Flow Cytometry : 1:10-1:50
IHC (Paraffin) : 1:50-1:100
Limitations This ABCG2 antibody is available for research use only.
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Description

ABCG2 is a membrane-associated protein included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue.

Application Notes

Titration of the ABCG2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 305-335 from the human protein was used as the immunogen for this ABCG2 antibody.

Storage

Aliquot the ABCG2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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