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Home >> Antibodies >> ABCB4 Antibody / MDR3

ABCB4 Antibody / MDR3 (R31688)

  Catalog No Formulation Size Price (USD)  
Image R31688 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Immunofluorescent staining of FFPE human U-2 OS with ABCB4 antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with ABCB4 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Flow cytometry testing of human HepG2 cells with ABCB4 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ABCB4 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P21439
Localization Cell membrane, cytoplasm
Applications IHC (FFPE) : 0.5-1ug/ml
Flow Cytometry : 1-3ug/million cells
Immunofluorescence : 2-4ug/ml
Limitations This ABCB4 antibody is available for research use only.
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Description

Adenosine triphosphate-binding cassette subfamily B, member 4, also called MDR3, is a protein that in humans is encoded by the ABCB4 gene. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABCB4 mutations represent a genetic factor involved in this peculiar form of cholesterol gallstone disease in adults.

Application Notes

Variations in secondary/substrate sensitivities and test protocols may require the ABCB4 antibody to be titrated for optimal performance.

Immunogen

Human partial recombinant protein (AA 601-720) was used as the immunogen for this ABCB4 antibody.

Storage

The lyophilized ABCB4 antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freeze/thaws.

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