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Home >> Antibodies >> AASS Antibody / LKR / SDH

AASS Antibody / LKR / SDH (RQ6595)

  Catalog No Formulation Size Price (USD)  
Image RQ6595 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 389
Immunofluorescent staining of FFPE human Caco-2 cells with AASS antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human liver cancer tissue with AASS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human kidney cancer tissue with AASS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HepG2, 2) human HEK293, 3) rat liver, 4) rat kidney, 5) mouse liver and 6) mouse kidney tissue lysate with AASS antibody. Predicted molecular weight ~102 kDa.
Flow cytometry testing of human Jurkat cells with AASS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AASS antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9UDR5
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This AASS antibody is available for research use only.
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Description

Alpha-aminoadipic semialdehyde synthase (AASS), also called Lysine ketoglutarate reductase (LKR) and Saccharopine dehydrogenase (SDH) is an enzyme encoded by the AASS gene in humans and is involved in their major lysine degradation pathway. This gene encodes a bifunctional enzyme that catalyzes the first two steps in the mammalian lysine degradation pathway. The N-terminal and the C-terminal portions of this enzyme contain lysine-ketoglutarate reductase and saccharopine dehydrogenase activity, respectively, resulting in the conversion of lysine to alpha-aminoadipic semialdehyde. Mutations in this gene are associated with familial hyperlysinemia.

Application Notes

Optimal dilution of the AASS antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids E37-N865) was used as the immunogen for the AASS antibody.

Storage

After reconstitution, the AASS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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