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Home >> Antibodies >> AAMP Antibody

AAMP Antibody (R31545)

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  Catalog No Formulation Size Price (USD)  
Image R31545 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of AAMP antibody and Lane 1: A431; 2: HeLa; 3: HEPG2; 4: MCF-7. Expected molecular weight: ~47 kDa.
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Western blot testing of AAMP antibody and Lane 1: A431; 2: HeLa; 3: HEPG2; 4: MCF-7. Expected molecular weight: ~47 kDa.
Western blot testing of AAMP antibody and recombinant human protein (0.5ng)
IHC-P: AAMP antibody testing of human breast cancer tissue
Immunofluorescent staining of FFPE human U-2 OS cells with AAMP antibody (red) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human K562 cells with AAMP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AAMP antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Gene ID 14
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This AAMP antibody is available for research use only.
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Description

Angio-associated migratory cell protein is in humans is encoded by the AAMP gene. It is mapped to 2q35. AAMP is an immunoglobulin-type protein which is found to be expressed strongly in endothelial cells, cytotrophoblasts, and poorly differentiated colon adenocarcinoma cells found in lymphatics. It has been demonstrated that an AAMP peptide containing the putative heparan sulfate-binding domain binds to heparin and mediates heparin-sensitive cell adhesion. It plays a role in angiogenesis and cell migration. In smooth muscle cell migration, it may act through the RhoA pathway.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the AAMP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 235-434) was used as the immunogen for this AAMP antibody.

Storage

After reconstitution, the AAMP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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