AACT Antibody for IHC / Alpha-1-antichymotrypsin Immunohistochemistry Antibody [clone SERPINA3/4184] (V9447)

AACT Antibody Protein Microarray Specificity Validation. Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using AACT antibody demonstrates highly specific binding to SERPINA3, with minimal off-target interaction across the array. The signal distribution shows a strong primary peak corresponding to SERPINA3, while all other proteins exhibit substantially lower signal intensity, supporting target selectivity of the antibody. Z-score represents the signal strength generated by antibody binding, expressed as standard deviations above the mean signal of the array, while S-score reflects the separation between the top-ranked target and the next highest signal, indicating relative specificity. The high Z-score and clear S-score separation observed for SERPINA3 confirm the specificity profile of this antibody clone in a proteome-wide context.

AACT Antibody for IHC Human Liver Tissue. Immunohistochemistry of Alpha-1-antichymotrypsin / SERPINA3 in FFPE human liver tissue using a mouse monoclonal AACT antibody, clone SERPINA3/4184. Strong HRP-DAB brown cytoplasmic staining is observed in hepatocytes, consistent with the secretory nature of this acute phase protein, while nuclei are counterstained blue. The staining pattern highlights widespread hepatocellular expression with minimal background signal. Heat induced epitope retrieval was performed by boiling sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling before staining.