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Home >> Antibodies >> a-Actin Antibody

a-Actin Antibody [clone 337CT30.10.1] (F53584)

  Catalog No Formulation Size Price (USD)  
Image F53584-0.1ML In ascites with 0.09% sodium azide 0.1 ml 329
a-Actin antibody western blot analysis in CEM lysate
Confocal immunofluorescent analysis of a-Actin antibody with A549 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
a-Actin antibody flow cytometric analysis of A549 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Bovine, Chicken, Mouse, Pig, Rabbit, Rat
Format Ascites
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name 337CT30.10.1
Purity Ascites
UniProt P68133
Applications Western blot : 1:1000-1:16000
Immunofluorescence : 1:10-1:50
Flow Cytometry : 1:10-1:50
Limitations This a-Actin antibody is available for research use only.
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Description

Actin is a highly conserved protein that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle.

Application Notes

Titration of the a-Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This a-Actin antibody was produced from a mouse immunized with ACTA1 recombinant protein.

Storage

Aliquot the a-Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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