LRP11 Antibody / LDL Receptor Related Protein 11 Antibody (FY13514)

LRP11 Antibody by IHC in Human Cerebellum Tissue. Immunohistochemistry staining of FFPE human cerebellum tissue using rabbit polyclonal LRP11 antibody at 2 ug/ml. Antigen retrieval was performed using EDTA buffer at pH 8.0, followed by blocking with 10% goat serum and overnight primary antibody incubation at 4oC. Detection was performed using peroxidase-conjugated goat anti-rabbit IgG and DAB. Brown LDL Receptor Related Protein 11 immunoreactivity is prominent within the cerebellar molecular layer and in large Purkinje neurons at the boundary with the granular layer. The densely packed cells of the granular layer exhibit comparatively weaker staining, highlighting regional differences in LRP11 immunoreactivity.

LRP11 Antibody by IHC in Human Brain Tissue. Immunohistochemistry staining of FFPE human brain tissue using rabbit polyclonal LRP11 antibody at 2 ug/ml. Heat-induced epitope retrieval was performed using EDTA buffer at pH 8.0. Tissue sections were blocked with 10% goat serum and incubated with primary antibody overnight at 4oC, followed by peroxidase-conjugated goat anti-rabbit IgG and DAB chromogenic detection. Brown immunoreactivity is distributed throughout the neuropil, with prominent staining of neuronal cell bodies and their processes. The predominantly cytoplasmic and process-associated staining pattern demonstrates the distribution of LDL Receptor Related Protein 11 immunoreactivity in human brain tissue.

LRP11 Antibody by IF in Human HeLa Cells. Immunofluorescence staining of human HeLa cells using rabbit polyclonal LRP11 antibody. LDL Receptor Related Protein 11 immunoreactivity is visualized in red, while nuclei are counterstained blue with DAPI. Red fluorescence is distributed predominantly throughout the cytoplasm, with a fine punctate and reticular appearance and areas of increased perinuclear staining. The nuclear regions show comparatively little red fluorescence. This staining pattern demonstrates intracellular LRP11 immunoreactivity in cultured human HeLa cells.

LRP11 Antibody by WB in Human HepG2, A431, HeLa and HaCaT Cells. Western blot analysis of human whole-cell lysates using rabbit polyclonal LRP11 antibody at 0.5 ug/ml. Lane 1: HepG2 cells; lane 2: A431 cells; lane 3: HeLa cells; lane 4: HaCaT cells. Samples containing 30 ug of protein per lane were separated by 10% SDS-PAGE under reducing conditions and transferred to a nitrocellulose membrane. Following overnight primary antibody incubation at 4oC, detection was performed using HRP-conjugated goat anti-rabbit IgG and chemiluminescence. A prominent band is detected at approximately 50 kDa in all four lysates, consistent with the calculated molecular weight of 53.3 kDa for LDL Receptor Related Protein 11. Additional weaker bands are visible above the principal band in some lanes.