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Home >> Antibodies >> 5-Lipoxygenase Antibody

5-Lipoxygenase Antibody (R30728)

  Catalog No Formulation Size Price (USD)  
Image R30728 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 419
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Western blot testing of 5-Lipoxygenase antibody and Lane 1: SW620; 2: Jurkat; 3: COLO320; 4: A549; 5: MCF-7 cell lysate. Predicted/observed size ~78KD
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P09917
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This 5-Lipoxygenase antibody is available for research use only.
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5-Lipoxygenase is an enzyme that in humans is encoded by the ALOX5 gene. It is a member of the lipoxygenase family of enzymes which also transforms EFAs into leukotrienes and is a current target for pharmaceutical intervention in a number of diseases. The enzyme 5-lipoxygenase catalyzes 2 reactions in the formation of leukotrienes. Human ALOX5 contains three nuclear localization sequences (NLSs) and a phosphorylation site involved in nuclear localization. Compared with age-matched competent mice, the knockout mice developed less right heart hypertrophy. Pharmacologic inhibition or gene disruption resulted in a significant decrease of beta-amyloid production and gamma-secretase levels. ALOX5 activity is short-lived, apparently in part because of an intrinsic instability of the enzyme.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the 5-Lipoxygenase antibody may be required due to differences in protocols and secondary/substrate sensitivity.


An amino acid sequence from the C-terminus of human ALOX5 (AERNKKKQLPYYYLSPDR) was used as the immunogen for this 5-Lipoxygenase antibody.


After reconstitution, the 5-Lipoxygenase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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