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Home >> Antibodies >> 4E-T Antibody / EIF4ENIF1

4E-T Antibody / EIF4ENIF1 (RQ7948)

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  Catalog No Formulation Size Price (USD)  
Image RQ7948 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Flow cytometry testing of human HL60 cells with 4E-T antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= 4E-T antibody.
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Flow cytometry testing of human HL60 cells with 4E-T antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= 4E-T antibody.
Western blot testing of 1) human HeLa, 2) human 293T, 3) human MCF7, 4) human HepG2, 5) rat brain, 6) rat NRK, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with 4E-T antibody. Expected molecular weight ~88/108 kDa (multiple isoforms but can be observed at ~140 kDa (possibly due to phosphorylation or high proline content-Ref 1).
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9NRA8
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This 4E-T antibody is available for research use only.
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  • Applications : WB, IHC, ELISA (peptide)
    Reactivity : Human
    Pred. Reactivity : Mouse, Rat, Dog, Pig, Cow

Description

Eukaryotic translation initiation factor 4E transporter (4E-T) is a protein that in humans is encoded by the EIF4ENIF1 gene. The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene.

Application Notes

Optimal dilution of the 4E-T antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids D13-Q985) was used as the immunogen for the 4E-T antibody.

Storage

After reconstitution, the 4E-T antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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