- Tel: 858.663.9055
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
17-beta-Hydroxysteroid dehydrogenase 4 Antibody (HSD17B4) (R30817)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
 |
R30817 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 429 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat |
| Format | Antigen affinity purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity |
| Buffer | Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal |
| UniProt | F5HE57 |
| Localization | Cytoplasmic |
| Applications | Western blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 0.5-1ug/ml |
| Limitations | This 17-beta-Hydroxysteroid dehydrogenase 4 antibody is available for research use only. |
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The HSD17B4 gene encodes an enzyme involved in peroxisomal fatty acid beta-oxidation. It was first identified as a 17-beta-estradiol dehydrogenase. Peroxisomal beta-oxidation of fatty acids is catalyzed by 3 enzymes: acyl-CoA oxidase; the D-bifunctional enzyme with enoyl-CoA-hydratase and D-3-hydroxyacyl-CoA dehydrogenase activity, and 3-ketoacyl-CoA thiolase. The D- and L-bifunctional proteins have different substrate specificities. The D-bifunctional protein catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids and also acts in shortening cholesterol for bile acid formation. In contrast, the L-specific bifunctional protein does not have the latter 2 activities.
The stated application concentrations are suggested starting amounts. Titration of the 17-beta-Hydroxysteroid dehydrogenase 4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
An amino acid sequence from the C-terminus of human HSD17B4 (NIMLSQKLQMILKDYAKL) was used as the immunogen for this 17-beta-Hydroxysteroid dehydrogenase 4 antibody (100% homologous in human, mouse and rat).
After reconstitution, the 17-beta-Hydroxysteroid dehydrogenase 4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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