TAGAP Antibody / T cell activation Rho GTPase activating protein [clone 30T40] (FY13222)
 Catalog No.  Formulation  Size
FY13222
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA
100 ul
Bulk quote request
Availability 2-3 weeks
Species Reactivity Human, Mouse, Rat
Format Liquid
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name 30T40
Purity Affinity chromatography
Buffer Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
UniProt Q8N103
Applications Western Blot : 1:500-1:2000
Immunoprecipitation : 1:50
Limitations This TAGAP antibody is available for research use only.
Western blot analysis of TAGAP using anti-TAGAP antibody. Lane 1: human Jurkat whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAGAP antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Western blot detection of TAGAP shows a single band at ~70 kDa across tested lysates. Despite a calculated mass of ~81 kDa, TAGAP frequently migrates lower on SDS-PAGE, consistent with isoform- and composition-dependent mobility.
Western blot analysis of TAGAP using anti-TAGAP antibody. Lane 1: human Jurkat whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAGAP antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Western blot detection of TAGAP shows a single band at ~70 kDa across tested lysates. Despite a calculated mass of ~81 kDa, TAGAP frequently migrates lower on SDS-PAGE, consistent with isoform- and composition-dependent mobility.