SRP54 Antibody / Signal recognition particle 54 kDa protein (FY13431)
 Catalog No.  Formulation  Size
FY13431
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
100 ug
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Availability 1-2 days
Species Reactivity Human, Mouse, Rat
Format Lyophilized
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Immunogen affinity purified
Buffer Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4
UniProt P61011
Localization Cytoplasm, ER, Nuclear speckles
Applications Western Blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Immunoprecipitation : 2ug per 500ug of lysate
Flow Cytometry : 1-3ug/million cells
Limitations This SRP54 antibody is available for research use only.
Immunofluorescent staining of FFPE human T-47D cells with SRP54 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human T-47D cells with SRP54 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot analysis using SRP54 antibody. Lane 1: human placenta tissue lysates; Lane 2: human HeLa whole cell lysates; Lane 3: human Jurkat whole cell lysates; Lane 4: human HEK293 whole cell lysates; Lane 5: rat stomach tissue lysates; Lane 6: mouse C6 whole cell lysates; Lane 7: mouse NIH/3T3 whole cell lysates. The predicted molecular weight of Signal recognition particle 54 kDa protein is ~56 kDa, and a band is observed at the expected size.
Western blot analysis using SRP54 antibody. Lane 1: human placenta tissue lysates; Lane 2: human HeLa whole cell lysates; Lane 3: human Jurkat whole cell lysates; Lane 4: human HEK293 whole cell lysates; Lane 5: rat stomach tissue lysates; Lane 6: mouse C6 whole cell lysates; Lane 7: mouse NIH/3T3 whole cell lysates. The predicted molecular weight of Signal recognition particle 54 kDa protein is ~56 kDa, and a band is observed at the expected size.
Immunoprecipitation of SRP54 protein from 500ug of human Jurkat whole cell lysate with 2ug of SRP54 antibody.
Immunoprecipitation of SRP54 protein from 500ug of human Jurkat whole cell lysate with 2ug of SRP54 antibody.
Flow cytometry analysis of fixed and permeabilized human U937 cells with SRP54 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SRP54 antibody.
Flow cytometry analysis of fixed and permeabilized human U937 cells with SRP54 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SRP54 antibody.