RNF4 Antibody / E3 Ubiquitin-Protein Ligase RNF4 Antibody (F54707)
 Catalog No.  Formulation  Size
F54707-0.4ML
In 1X PBS, pH 7.4, with 0.09% sodium azide
0.4 ml
F54707-0.08ML
In 1X PBS, pH 7.4, with 0.09% sodium azide
0.08 ml
./plp/all_image/img-F54707-0.4ML-1.jpg ./plp/all_image/img-F54707-0.4ML-2.jpg ./plp/all_image/img-F54707-0.4ML-3.jpg ./plp/all_image/img-F54707-0.4ML-4.jpg
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Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt P78317
Localization Cytoplasmic, nuclear
Applications Western Blot : 1:500-1:2000
Flow Cytometry : 1:25 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:25
Limitations This RNF4 antibody is available for research use only.
RNF4 Antibody / E3 Ubiquitin-Protein Ligase RNF4 Antibody. Immunohistochemistry analysis of RNF4 in FFPE human heart tissue. IHC staining of FFPE human heart using RNF4 Antibody / E3 Ubiquitin-Protein Ligase RNF4 Antibody demonstrates HRP-DAB brown staining within cardiomyocytes, with signal primarily localized to intracellular compartments consistent with nuclear and perinuclear distribution of this E3 ubiquitin ligase. The staining pattern reflects cellular regions associated with ubiquitin-mediated protein regulation and SUMO-targeted ubiquitination activity, while surrounding tissue architecture remains clearly defined.
RNF4 Antibody / E3 Ubiquitin-Protein Ligase RNF4 Antibody. Immunohistochemistry analysis of RNF4 in FFPE human heart tissue. IHC staining of FFPE human heart using RNF4 Antibody / E3 Ubiquitin-Protein Ligase RNF4 Antibody demonstrates HRP-DAB brown staining within cardiomyocytes, with signal primarily localized to intracellular compartments consistent with nuclear and perinuclear distribution of this E3 ubiquitin ligase. The staining pattern reflects cellular regions associated with ubiquitin-mediated protein regulation and SUMO-targeted ubiquitination activity, while surrounding tissue architecture remains clearly defined.
RNF4 Antibody / E3 Ubiquitin-Protein Ligase RNF4 Antibody for WB. Western blot analysis of RNF4 in human kidney tissue lysate. Lane 1: human kidney tissue lysate. A band is detected at approximately 28 kDa, consistent with the predicted molecular weight of E3 ubiquitin-protein ligase RNF4 (isoform 1). The observed signal aligns with expression of this nuclear E3 ubiquitin ligase, which functions in SUMO-targeted ubiquitination and regulation of protein degradation pathways.
Flow cytometry testing of fixed and permeabilized human HepG2 cells with RNF4 Antibody / E3 Ubiquitin-Protein Ligase RNF4 Antibody; Blue=isotype control, Green= RNF4 antibody.
Western blot testing of human LNCaP cell lysate with RNF4 antibody. Expected molecular weight: 15-30 kDa (isoform 1).
Western blot testing of human LNCaP cell lysate with RNF4 antibody. Expected molecular weight: 15-30 kDa (isoform 1).
RNF4 Antibody / E3 Ubiquitin-Protein Ligase RNF4 Antibody for FACS. Flow cytometry analysis of RNF4 in permeabilized human HepG2 cells. Flow cytometric analysis of fixed and permeabilized human HepG2 cells stained with RNF4 Antibody / E3 Ubiquitin-Protein Ligase RNF4 Antibody shows a clear rightward shift of the RNF4-positive population (green) relative to isotype control (blue). The signal reflects intracellular detection of this nuclear E3 ubiquitin ligase, consistent with its role in SUMO-targeted ubiquitination and regulation of protein turnover pathways.
RNF4 Antibody / E3 Ubiquitin-Protein Ligase RNF4 Antibody for FACS. Flow cytometry analysis of RNF4 in permeabilized human HepG2 cells. Flow cytometric analysis of fixed and permeabilized human HepG2 cells stained with RNF4 Antibody / E3 Ubiquitin-Protein Ligase RNF4 Antibody shows a clear rightward shift of the RNF4-positive population (green) relative to isotype control (blue). The signal reflects intracellular detection of this nuclear E3 ubiquitin ligase, consistent with its role in SUMO-targeted ubiquitination and regulation of protein turnover pathways.