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Immunohistochemistry analysis of LFA-2 antibody (clone rLFA2/8516) in human tonsil tissue. Formalin-fixed, paraffin-embedded tonsil shows strong membranous HRP-DAB brown staining in interfollicular T lymphocytes, consistent with CD2 expression on mature T cells. Germinal center regions display comparatively reduced staining, highlighting the expected distribution of T cell populations within lymphoid tissue. Hematoxylin counterstain clearly delineates nuclear morphology and overall tonsillar architecture. The inset shows PBS used in place of primary antibody as a negative control, confirming absence of non-specific secondary antibody binding. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 20 minutes followed by cooling prior to staining.
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