NDUFS8 Antibody / NADH-ubiquinone oxidoreductase 23 kDa subunit (RQ7236)
 Catalog No.  Formulation  Size
RQ7236
0.5mg/ml if reconstituted with 0.2ml sterile DI water
100 ug
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Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt O00217
Localization Cytoplasmic (mitochondria)
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow Cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This NDUFS8 antibody is available for research use only.
Western blot testing of 1) rat heart, 2) rat liver, 3) rat skeletal muscle, 4) mouse heart, 5) mouse liver, 6) mouse skeletal muscle and 7) mouse C2C12 cell lysate with NDUFS8 antibody. Expected molecular weight ~23 kDa.
Western blot testing of 1) rat heart, 2) rat liver, 3) rat skeletal muscle, 4) mouse heart, 5) mouse liver, 6) mouse skeletal muscle and 7) mouse C2C12 cell lysate with NDUFS8 antibody. Expected molecular weight ~23 kDa.
Western blot testing of human 1) HeLa, 2) HepG2, 3) A549, 4) MCF7, 5) Caco-2, 6) PC-3 and 7) 293T cell lysate with NDUFS8 antibody. Expected molecular weight ~23 kDa.
Western blot testing of human 1) HeLa, 2) HepG2, 3) A549, 4) MCF7, 5) Caco-2, 6) PC-3 and 7) 293T cell lysate with NDUFS8 antibody. Expected molecular weight ~23 kDa.
IHC staining of FFPE human liver cancer tissue with NDUFS8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with NDUFS8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse heart tissue with NDUFS8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse heart tissue with NDUFS8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human liver cancer tissue with NDUFS8 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human liver cancer tissue with NDUFS8 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Flow cytometry testing of human 293T cells with NDUFS8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NDUFS8 antibody.
Flow cytometry testing of human 293T cells with NDUFS8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NDUFS8 antibody.
Flow cytometry testing of human HeLa cells with NDUFS8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NDUFS8 antibody.
Flow cytometry testing of human HeLa cells with NDUFS8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NDUFS8 antibody.