Western Blot : 0.5-1ug/ml Immunofluorescence : 5ug/ml Flow Cytometry : 1-3ug/million cells ELISA : 0.1-0.5ug/ml
Limitations
This LDOC1 antibody is available for research use only.
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Immunofluorescent staining of FFPE human HeLa cells with LDOC1 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of human HeLa cell lysate with LDOC1 antibody. Predicted molecular weight ~17 kDa.
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Flow cytometry testing of fixed and permeabilized human U-251 cells with LDOC1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LDOC1 antibody.