Western Blot : 0.5-1ug/ml Flow Cytometry : 1-3ug/million cells Direct ELISA : 0.1-0.5ug/ml
Limitations
This ICAM2 antibody is available for research use only.
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Flow cytometry testing of fixed human HEL cells with ICAM2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ICAM2 antibody.
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Western blot testing of 1) human Jurkat, 2) human HEL, 3) human K562 and 4) mouse spleen tissue lysate with ICAM2 antibody at 0.5ug/ml. Expected molecular weight: 31-60 kDa depending on glycosylation level.