Grancalcin Antibody / GCA (RQ4647)
 Catalog No.  Formulation  Size
RQ4647
0.5mg/ml if reconstituted with 0.2ml sterile DI water
100 ug
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Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P28676
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFEP) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow Cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml (human recombinant protein)
Limitations This Grancalcin antibody is for research use only.
Western blot testing of human 1) placenta, 2) HL-60 and 3) Caco-2 lysate with Grancalcin antibody at 0.5ug/ml. Predicted molecular weight ~24 kDa.
Western blot testing of human 1) placenta, 2) HL-60 and 3) Caco-2 lysate with Grancalcin antibody at 0.5ug/ml. Predicted molecular weight ~24 kDa.
Western blot testing of 1) human MCF7, 2) human HL-60, 3) human HeLa, 4) rat testis and 5) mouse testis tissue lysate with Grancalcin antibody at 0.5ug/ml. Predicted molecular weight ~24 kDa.
Western blot testing of 1) human MCF7, 2) human HL-60, 3) human HeLa, 4) rat testis and 5) mouse testis tissue lysate with Grancalcin antibody at 0.5ug/ml. Predicted molecular weight ~24 kDa.
IHC staining of FFPE human bladder urothelial carcinoma tissue with Grancalcin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human bladder urothelial carcinoma tissue with Grancalcin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with Grancalcin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with Grancalcin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with Grancalcin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with Grancalcin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HeLa cells with Grancalcin antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human HeLa cells with Grancalcin antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human HL60 cells with Grancalcin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Grancalcin antibody.
Flow cytometry testing of human HL60 cells with Grancalcin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Grancalcin antibody.