DLAT Antibody / Pyruvate Dehydrogenase E2 (RQ6548)
 Catalog No.  Formulation  Size
RQ6548
0.5mg/ml if reconstituted with 0.2ml sterile DI water
100 ug
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Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P10515
Localization Cytoplasmic
Applications Western Blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow Cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This DLAT antibody is available for research use only.
IHC staining of FFPE human liver cancer tissue with DLAT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with DLAT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal clear cell carcinoma tissue with DLAT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal clear cell carcinoma tissue with DLAT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with DLAT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with DLAT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with DLAT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with DLAT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with DLAT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with DLAT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human Caco-2 cells with DLAT antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human Caco-2 cells with DLAT antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) HepG2, 3) K562 and 4) T-47D cell lysate with DLAT antibody. Predicted molecular weight ~69 kDa.
Western blot testing of human 1) HeLa, 2) HepG2, 3) K562 and 4) T-47D cell lysate with DLAT antibody. Predicted molecular weight ~69 kDa.
Western blot testing of 1) rat brain, 2) rat kidney, 3) mouse brain, 4) mouse kidney, 5) mouse testis and 6) mouse NIH 3T3 cell lysate with DLAT antibody. Predicted molecular weight ~69 kDa.
Western blot testing of 1) rat brain, 2) rat kidney, 3) mouse brain, 4) mouse kidney, 5) mouse testis and 6) mouse NIH 3T3 cell lysate with DLAT antibody. Predicted molecular weight ~69 kDa.
Flow cytometry testing of human A431 cells with DLAT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DLAT antibody.
Flow cytometry testing of human A431 cells with DLAT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DLAT antibody.