CD59 Antibody (R31963)
 Catalog No.  Formulation  Size
R31963
0.5mg/ml if reconstituted with 0.2ml sterile DI water
100 ug
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Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt P13987
Localization Plasma membrane, cytoplasm
Applications Immunohistochemistry (FFPE) : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow Cytometry : 1-3ug/10^6 cells
Western Blot : 1-2ug/ml
Limitations This CD59 antibody is available for research use only.
Immunofluorescent staining of FFPE human A431 cells with CD59 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human A431 cells with CD59 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human K562 cells with CD59 antibody at 1ug/10^6 cells; Red=cells alone, Green=isotype control, Blue=CD59 antibody.
Flow cytometry testing of human K562 cells with CD59 antibody at 1ug/10^6 cells; Red=cells alone, Green=isotype control, Blue=CD59 antibody.
IHC testing of FFPE human tonsil with CD59 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE human tonsil with CD59 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Western blot testing of human 1) HeLa and 2) PC-3 cell lysate with CD59 antibody. Expected molecular weight: 14-20 kDa depending on level of glycosylation.
Western blot testing of human 1) HeLa and 2) PC-3 cell lysate with CD59 antibody. Expected molecular weight: 14-20 kDa depending on level of glycosylation.