ATXN3 Antibody (R32137)
 Catalog No.  Formulation  Size
R32137
0.5mg/ml if reconstituted with 0.2ml sterile DI water
100 ug
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Availability 1-3 business days
Species Reactivity Human, Rat
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P54252
Localization Nuclear and cytoplasmic
Applications Western Blot : 0.1-0.5ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Flow Cytometry : 1-3ug/million cells
Immunofluorescence : 2-4ug/ml
Limitations This ATXN3 antibody is available for research use only.
Western blot testing of 1) rat brain, 2) human COLO320 and 3) human HeLa lysate with ATXN3 antibody. Predicted/observed molecular weight ~42 kDa.
Western blot testing of 1) rat brain, 2) human COLO320 and 3) human HeLa lysate with ATXN3 antibody. Predicted/observed molecular weight ~42 kDa.
IHC testing of FFPE human lung cancer with ATXN3 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE human lung cancer with ATXN3 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Immunofluorescent staining of FFPE human MCF7 cells with ATXN3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human MCF7 cells with ATXN3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A549 cells with ATXN3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATXN3 antibody.
Flow cytometry testing of human A549 cells with ATXN3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATXN3 antibody.
Flow cytometry testing of human SiHa cells with ATXN3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATXN3 antibody.
Flow cytometry testing of human SiHa cells with ATXN3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATXN3 antibody.