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Home >> Antibodies >> ZNF774 Antibody / Zinc finger protein 774

ZNF774 Antibody / Zinc finger protein 774 [clone PCRP-ZNF774-3F7] (V4397)

  Catalog No Formulation Size Price (USD)  
Image V4397-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4397-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4397SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated
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Flow cytometry testing of PFA-fixed human HeLa cells with ZNF774 antibody (clone PCRP-ZNF774-3F7) followed by goat anti-mouse IgG-CF488 (blue); Red = unstained cells.
SDS-PAGE analysis of purified, BSA-free ZNF774 antibody (clone PCRP-ZNF774-3F7) as confirmation of integrity and purity.
Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using ZNF774 antibody (clone PCRP-ZNF774-3F7). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG
Clone Name PCRP-ZNF774-3F7
Purity Protein A/G affinity
UniProt Q6NX45
Localization Nucleus
Applications ELISA (Order BSA-free format for coating) :
Flow cytometry : 0.5-2ug/million cells
Limitations This ZNF774 antibody is available for research use only.
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Description

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Apr 2022]

Application Notes

Optimal dilution of the ZNF774 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human ZNF774 protein was used as the immunogen for the ZNF774 antibody.

Storage

Aliquot the ZNF774 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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