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Home >> Antibodies >> Zebrafish Rab3Gap1 Antibody

Zebrafish Rab3Gap1 Antibody (RZ1056)

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  Catalog No Formulation Size Price (USD)  
Image RZ1056 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 539
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IHC staining of FFPE zebrafish kidney tissue with Zebrafish Rab3Gap1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE zebrafish kidney tissue with Zebrafish Rab3Gap1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot analysis of Rab3Gap1 protein using Zebrafish Rab3Gap1 antibody and 1) zebrafish head and 2) zebrafish embryo tissue lysate. Predicted molecular weight ~109 kDa, commonly observed at ~130 kDa.
IHC staining of FFPE zebrafish brain tissue with Zebrafish Rab3Gap1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE zebrafish colon tissue with Zebrafish Rab3Gap1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE zebrafish heart tissue with Zebrafish Rab3Gap1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE zebrafish liver tissue with Zebrafish Rab3Gap1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE zebrafish spleen tissue with Zebrafish Rab3Gap1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 2-3 weeks
Species Reactivity Zebrafish
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q6NUV0
Localization Cytoplasm
Applications Western Blot : 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 2-5 ug/ml
Limitations This Zebrafish Rab3Gap1 antibody is available for research use only.
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Description

Rab3 GTPase-activating protein catalytic subunit is an enzyme that in humans is encoded by the RAB3GAP1 gene. This gene encodes the catalytic subunit of a Rab GTPase activating protein. The encoded protein forms a heterodimer with a non-catalytic subunit to specifically regulate the activity of members of the Rab3 subfamily of small G proteins. This protein mediates the hydrolysis of GTP bound Rab3 to the GDP bound form. Mutations in this gene are associated with Warburg micro syndrome. Alternate splicing results in multiple transcript variants.

Application Notes

Optimal dilution of the Zebrafish Rab3Gap1 antibody should be determined by the researcher.

Immunogen

An E.coli-derived zebrafish Rab3Gap1 recombinant protein (amino acids N109-Q798) was used as the immunogen for the Zebrafish Rab3Gap1 antibody.

Storage

After reconstitution, the Zebrafish Rab3Gap1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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