- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Zebrafish Gnao1a Antibody / Guanine nucleotide-binding protein G(o) subunit alpha (RZ1172)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RZ1172 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 539 |
| Availability | 2-3 weeks |
| Species Reactivity | Zebrafish |
| Format | Antigen affinity purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Purity | Antigen affinity chromatography |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | Q6PBP1 |
| Localization | Cytoplasm, cell membrane |
| Applications | Western Blot : 0.5-1 ug/ml Immunohistochemistry (FFPE) : 2-5 ug/ml Immunofluorescence : 5 ug/ml |
| Limitations | This Zebrafish Gnao1a antibody is available for research use only. |
|
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Zebrafish (Danio rerio) Gnao1a antibody detects Gnao1a, a G alpha subunit that mediates signaling by inhibitory G protein-coupled receptors (GPCRs). Encoded in zebrafish by the gnao1a gene, Guanine nucleotide-binding protein Go subunit alpha regulates neuronal excitability, synaptic transmission, vesicle release, and developmental signaling pathways. Go proteins represent one of the most abundant G alpha subtypes in the vertebrate nervous system, and their activity modulates ion channels, second-messenger pathways, and cytoskeletal dynamics. Because these signaling processes shape early neural patterning and circuit function, Zebrafish Gnao1a antibody reagents support research in GPCR signaling, neurodevelopment, and synaptic physiology.
Go is activated when GPCRs promote GDP-GTP exchange on the Gnao1a subunit, allowing dissociation of G alpha and G beta gamma complexes. These components regulate effectors including voltage-gated calcium channels, inward-rectifier potassium channels, adenylyl cyclases, and regulators of vesicle trafficking. In zebrafish embryos, gnao1a expression is enriched in developing neural tissues such as the hindbrain, spinal cord, retina, and cranial sensory structures. These regions rely on Go-dependent modulation of membrane excitability and neurotransmitter release to refine early neuronal connectivity.
During neurodevelopment, Go signaling influences axon guidance, synaptic maturation, and activity-dependent plasticity. Gnao1a helps tune firing thresholds and enhance or dampen synaptic communication depending on receptor context. Zebrafish provide a powerful model for observing these processes, as gnao1a-dependent phenotypes can alter locomotor behaviors, sensory processing, or neuronal patterning. Because Go is a major inhibitory regulator, disruptions in its activity can lead to excessive neurotransmission, impaired circuit formation, or altered developmental timing.
Beyond synaptic roles, Guanine nucleotide-binding protein Go subunit alpha participates in GPCR pathways that regulate cell migration, proliferation, and morphogenetic movements during embryogenesis. In vertebrates, Go has been implicated in controlling cytoskeletal remodeling and guidance cues that position neurons and glial cells. Similar mechanisms likely occur in zebrafish, where coordinated cell movements shape the neural tube, craniofacial structures, and sensory ganglia.
At the molecular level, Gnao1a contains conserved GTP-binding motifs and switch regions that undergo conformational changes when activated. These changes allow Go to regulate its effectors and reassemble with G beta gamma subunits following GTP hydrolysis. Subcellular localization is primarily at the plasma membrane, but Go can also associate with intracellular membranes and vesicular compartments involved in neurotransmitter release and endocytosis.
Go signaling interacts with broader networks including MAPK, calcium signaling, and cAMP pathways. These interactions help integrate extracellular cues with intracellular responses that guide neuronal differentiation and synaptic refinement. Because zebrafish embryos develop rapidly, proper Go function ensures that signaling thresholds are maintained throughout dynamic developmental transitions.
A Zebrafish Gnao1a antibody is suitable for research applications such as western blotting, immunohistochemistry, and assays examining GPCR signaling, neuronal maturation, synaptic function, and developmental patterning. This antibody targets Guanine nucleotide-binding protein Go subunit alpha for studies involving inhibitory G protein signaling and vertebrate neurobiology. NSJ Bioreagents provides the Zebrafish Gnao1a antibody to support research in neuronal signaling and developmental physiology.
Optimal dilution of the Zebrafish Gnao1a antibody should be determined by the researcher.
An E.coli-derived zebrafish Gnao1a recombinant protein (amino acids C3-R179) was used as the immunogen for the Zebrafish Gnao1a antibody.
After reconstitution, the Zebrafish Gnao1a antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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