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Home >> Antibodies >> ZBTB16 Antibody / PLZF

ZBTB16 Antibody / PLZF (RQ5786)

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  Catalog No Formulation Size Price (USD)  
Image RQ5786 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human A431 cells with ZBTB16 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human A431 cells with ZBTB16 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HL-60, 2) HEK293, 3) ThP-1, 4) HeLa, 5) Raji, 6) K562, 7) rat spleen, 8) mouse RAW264.7 lysate with ZBTB16 antibody. Predicted molecular weight: 74-81 kDa.
Flow cytometry testing of human 293T cells with ZBTB16 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ZBTB16 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q05516
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This ZBTB16 antibody is available for research use only.
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Description

Zinc finger and BTB domain-containing protein 16 is a protein that in humans is encoded by the ZBTB16 gene. This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized.

Application Notes

Optimal dilution of the ZBTB16 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids K23-V673) was used as the immunogen for the ZBTB16 antibody.

Storage

After reconstitution, the ZBTB16 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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