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Home >> Antibodies >> XRCC1 Antibody

XRCC1 Antibody (F49231)

  Catalog No Formulation Size Price (USD)  
Image F49231-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F49231-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Immunofluorescent analysis of A549 cells using XRCC1 antibody at 1:25. Alexa Fluor 488-conjugated secondary was used (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 conjugated Phalloidin (red).
Immunofluorescent analysis of A549 cells using XRCC1 antibody at 1:25. Alexa Fluor 488-conjugated secondary was used (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 conjugated Phalloidin (red).
XRCC1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma.
XRCC1 antibody western blot analysis in A375 lysate. Rountinely observed molecular weight: 69~90 kDa.
XRCC1 antibody flow cytometric analysis of A375 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P18887
Localization Nuclear
Applications Immunofluorescence : 1:25
Western blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Flow Cytometry : 1:10-1:50
Limitations This XRCC1 antibody is available for research use only.
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Description

The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

Application Notes

Titration of the XRCC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 407-435 from the human protein was used as the immunogen for this XRCC1 antibody.

Storage

Aliquot the XRCC1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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