WT1-associated protein Antibody / WTAP [clone PCRP-WTAP-1A4] (V5193)

IHC staining of FFPE human colon carcinoma tissue with WT1-associated protein antibody (clone PCRP-WTAP-1A4). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Flow cytometry testing of PFA-fixed human HeLa cells with WT1-associated protein antibody (clone PCRP-WTAP-1A4) followed by goat anti-mouse IgG-CF488 (blue); Red = unstained cells.

Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using WT1-associated protein antibody (PCRP-WTAP-1A4). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.