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Home >> Antibodies >> WEE1 Antibody

WEE1 Antibody (RQ5782)

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  Catalog No Formulation Size Price (USD)  
Image RQ5782 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human A549 cells with WEE1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= WEE1 antibody.
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Flow cytometry testing of human A549 cells with WEE1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= WEE1 antibody.
Western blot testing of 1) human HeLa, 2) human K562, 3) mouse lung and 4) mouse kidney lysate with WEE1 antibody. Predicted molecular weight ~72 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P30291
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This WEE1 antibody is available for research use only.
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Description

Wee1 is a nuclear kinase belonging to the Ser/Thr family of protein kinases in the fission yeast Schizosaccharomyces pombe (S. pombe). I t is mapped to 11p15.4. This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

Application Notes

Optimal dilution of the WEE1 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids R13-R581) was used as the immunogen for the WEE1 antibody.

Storage

After reconstitution, the WEE1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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