- Tel: 858.663.9055
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Wee1 Antibody (F54097)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
 |
F54097-0.2ML | In 1X PBS, pH 7.4, with 0.09% sodium azide | 0.2 ml | 399 | |
|
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F54097-0.05ML | In 1X PBS, pH 7.4, with 0.09% sodium azide | 0.05 ml | 185 |
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| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Antigen affinity purified |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Purity | Antigen affinity |
| UniProt | P30291 |
| Applications | Western Blot : 1:2000 |
| Limitations | This Wee1 antibody is available for research use only. |
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Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. [UniProt]
The stated application concentrations are suggested starting points. Titration of the Wee1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 96-130 from human Wee1 was used as the immunogen for the Wee1 antibody.
Aliquot the Wee1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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