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Home >> Antibodies >> Vimentin Antibody

Vimentin Antibody [clone V9] (V8160)

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  Catalog No Formulation Size Price (USD)  
Image V8160-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8160-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8160SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Western blot testing of human U-87 cell lysate with Vimentin antibody (clone V9). Predicted molecular weight ~53 kDa.
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Western blot testing of human U-87 cell lysate with Vimentin antibody (clone V9). Predicted molecular weight ~53 kDa.
IHC staining of FFPE human uterine carcinoma with Vimentin antibody (clone V9). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Vimentin antibody (clone V9) as confirmation of integrity and purity.
IHC staining of FFPE human tonsil with Vimentin antibody (clone V9). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name V9
Purity Protein G affinity chromatography
UniProt P08670
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Vimentin antibody is available for research use only.
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Description

This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Application Notes

Optimal dilution of the Vimentin antibody should be determined by the researcher.

Immunogen

Vimentin protein purified from porcine eye lens cells was used as the immunogen for this Vimentin antibody.

Storage

Store the Vimentin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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