• Tel: 858.663.9055
  • Email: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> Vimentin Antibody

Vimentin Antibody (R31772)

  Catalog No Formulation Size Price (USD)  
R31772 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 299
Western blot testing of Vimentin antibody and Lane 1: (h) HT1080; 2: (m) NIH3T3; 3: (h) Jurkat; 4: (h) HUT; 5: (h) MCF-7; 6: (h) HeLa; 7: human placenta; 8: rat testis; 9: mouse testis lysate. Predicted molecular weight ~54 kDa.
IHC-P: Vimentin antibody testing of human breast cancer tissue. Required HIER: Boil the paraffin sections in 10mM citrate buffer, pH6, for 20 min.
IHC-P: Vimentin antibody testing of mouse heart. Required HIER: Boil the paraffin sections in 10mM citrate buffer, pH6, for 20 min.
IHC-P: Vimentin antibody testing of rat heart. Required HIER: Boil the paraffin sections in 10mM citrate buffer, pH6, for 20 min.
IHC-P: Vimentin antibody testing of rat kidney. Required HIER: Boil the paraffin sections in 10mM citrate buffer, pH6, for 20 min.
IHC-P: Vimentin antibody testing of mouse kidney. Required HIER: Boil the paraffin sections in 10mM citrate buffer, pH6, for 20 min.
Western blot testing of Vimentin antibody and 1) rat kidney and 2) mouse kidney lysate. Predicted molecular weight ~54 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Gene ID 7431
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This Vimentin antibody is available for research use only.

Related Products

Description

Vimentin, also known as HEL113 or CTRCT30, is encoded by the VIM gene and is a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminus of human Vimentin (DTHSKRTLLIKTVETRDGQVINETSQHHDDLE) was used as the immunogen for this Vimentin antibody (100% homologous in human, mouse and rat).

Storage

After reconstitution, the Vimentin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.