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Home >> Antibodies >> Vimentin Antibody

Vimentin Antibody [clone 4F8-A5-H5] (F54035)

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  Catalog No Formulation Size Price (USD)  
Image F54035-0.1ML In PBS with 50% glycerol and 0.03% ProClin 300 0.1 ml 399
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Western blot testing of Vimentin-positive cell lines (Molt-4, K562, COS7, Jurkat, HeLa) and Vimentin-negative cell lines (Daudi, Ramos, Raji) using Vimentin antibody at 1:1000. Predicted molecular weight ~58 kDa.
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Western blot testing of Vimentin-positive cell lines (Molt-4, K562, COS7, Jurkat, HeLa) and Vimentin-negative cell lines (Daudi, Ramos, Raji) using Vimentin antibody at 1:1000. Predicted molecular weight ~58 kDa.
Immunoprecipitation of Vimentin from HeLa cell lysate, and subsequent western blot testing, using Vimentin antibody.
ICC/IF staining of HeLa cells, fixed with 4% Paraformaldehyde, using Vimentin antibody at 1:800.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a
Clone Name 4F8-A5-H5
Purity Protein G affinity
UniProt P08670
Applications Western blot : 1:1000
ICC/IF : 1:800
Immunoprecipitation : suitable
Limitations This Vimentin antibody is available for research use only.
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Description

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A human recombinant partial protein was used as the immunogen for this Vimentin antibody.

Storage

Store the Vimentin antibody at -20oC.

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