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Home >> Antibodies >> VIM Antibody / Vimentin

VIM Antibody / Vimentin [clone AGF-22] (RQ5250)

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Image RQ5250 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 429
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IHC staining of FFPE human colon tissue with VIM antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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IHC staining of FFPE human colon tissue with VIM antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of human HEK293 cell lysate with VIM antibody. Expected molecular weight: 53-58 kDa.
Immunofluorescent staining of human HeLa cells with VIM antibody (green) and DAPI nuclear stain (blue).
Availability 1-2 weeks
Species Reactivity Human
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name AGF-22
Purity Affinity purified
UniProt P08670
Localization Cytoplasmic, nuclear, cell membrane
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:50-1:200
Immunofluorescence : 1:50-1:200
Limitations This VIM antibody is available for research use only.
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Description

The VIM gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. [RefSeq]

Application Notes

Optimal dilution of the VIM antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human Vimentin / VIM was used as the immunogen for the VIM antibody.

Storage

Store the VIM antibody at -20oC.

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