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Home >> Antibodies >> Villin Antibody / VIL1

Villin Antibody / VIL1 [clone VIL1/7378R] (V4949)

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  Catalog No Formulation Size Price (USD)  
Image V4949-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4949-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4949SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human colon adenocarcinoma with Villin antibody (clone VIL1/7378R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human colon adenocarcinoma with Villin antibody (clone VIL1/7378R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon adenocarcinoma with Villin antibody (clone VIL1/7378R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name VIL1/7378R
Purity Protein A/G affinity
UniProt P09327
Localization Cytoplasm, Cell Surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Villin antibody is available for research use only.
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Description

Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

Application Notes

Optimal dilution of the Villin antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 600-700) from the human protein was used as the immunogen for the Villin antibody.

Storage

Aliquot the Villin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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