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Home >> Antibodies >> VIL1 Antibody / Villin

VIL1 Antibody / Villin [clone rVIL1/8336] (V4947)

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  Catalog No Formulation Size Price (USD)  
Image V4947-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4947-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4947SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human colon adenocarcinoma with VIL1 antibody (clone rVIL1/8336). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon adenocarcinoma with VIL1 antibody (clone rVIL1/8336). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rVIL1/8336
Purity Protein A/G affinity
UniProt P09327
Localization Cytoplasm, Cell Surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This VIL1 antibody is available for research use only.
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Description

Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

Application Notes

Optimal dilution of the VIL1 antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 600-700) from the human protein was used as the immunogen for the VIL1 antibody.

Storage

Aliquot the VIL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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