Vegf receptor 2 Antibody / Vegfr2 (RQ4055)

IHC testing of FFPE rat kidney tissue with Vegf receptor 2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IHC testing of FFPE mouse kidney tissue with Vegf receptor 2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Western blot testing of recombinant mouse Vegfr2 protein with Vegf receptor 2 antibody at 0.5ug/ml.

Western blot testing of mouse 1) heart, 2) thymus, 3) spleen, 4) kidney, 5) brain, 6) lung, 7) HEPA1-6 and 8) NIH 3T3 lysate with Vegf receptor 2 antibody. Predicted molecular weight: ~152 (immature), 180-200 kDa (intermediate) and 220-230 kDa (mature).

Immunofluorescent staining of FFPE human NIH 3T3 cells with Vegf receptor 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Flow cytometry testing of human MCF7 cells with Vegf receptor 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Vegf receptor 2 antibody.

Flow cytometry testing of human LLC1 cells with Vegf receptor 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Vegf receptor 2 antibody.