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Home >> Antibodies >> VEGF Antibody / Vascular Endothelial Growth Factor

VEGF Antibody / Vascular Endothelial Growth Factor [clone VEGFA/7635R] (V5114)

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  Catalog No Formulation Size Price (USD)  
Image V5114-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V5114-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V5114SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human placental tissue with VEGF antibody (clone VEGFA/7635R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human placental tissue with VEGF antibody (clone VEGFA/7635R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver tissue with VEGF antibody (clone VEGFA/7635R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human heart tissue with VEGF antibody (clone VEGFA/7635R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG1, kappa
Clone Name VEGFA/7635R
Purity Protein A/G affinity
UniProt P15692
Localization Cytoplasm, Cell surface, Extracellular (secreted)
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This VEGF antibody is available for research use only.
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Description

This mAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

Application Notes

Optimal dilution of the VEGF antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the VEGF antibody.

Storage

Aliquot the VEGF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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