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Home >> Antibodies >> VASP Antibody

VASP Antibody (R32632)

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  Catalog No Formulation Size Price (USD)  
Image R32632 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC testing of FFPE human lung cancer tissue with VASP antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
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IHC testing of FFPE human lung cancer tissue with VASP antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human breast cancer tissue with VASP antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) rat liver, 2) mouse kidney, 3) human HeLa and 4) human HePG2 lysate with VASP antibody at 0.5ug/ml. Expected molecular weight: ~40/46-50 kDa (unmodified/phosphorylated).
Flow cytometry testing of human SiHa cells with VASP antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VASP antibody.
Flow cytometry testing of human U937 cells with VASP antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VASP antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P50552
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Flow cytometry : 1-3ug/10^6 cells
Limitations This VASP antibody is available for research use only.
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Description

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.

Application Notes

Optimal dilution of the VASP antibody should be determined by the researcher.

Immunogen

Amino acids 78-114 (NFHQWRDARQVWGLNFGSKEDAAQFAAGMASALEALE-human) were used as the immunogen for this VASP antibody.

Storage

After reconstitution, the VASP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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