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Home >> Antibodies >> Vascular Endothelial Growth Factor Antibody / VEGF

Vascular Endothelial Growth Factor Antibody / VEGF [clone VEGFA/7757R] (V5095)

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  Catalog No Formulation Size Price (USD)  
Image V5095-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V5095-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V5095SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human placental tissue with Vascular Endothelial Growth Factor antibody (clone VEGFA/7757R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human placental tissue with Vascular Endothelial Growth Factor antibody (clone VEGFA/7757R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with Vascular Endothelial Growth Factor antibody (clone VEGFA/7757R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG1, kappa
Clone Name VEGFA/7757R
Purity Protein A/G affinity
UniProt P15692
Localization Cytoplasm, Cell surface, Extracellular (secreted)
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Vascular Endothelial Growth Factor antibody is available for research use only.
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Description

This mAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

Application Notes

Optimal dilution of the Vascular Endothelial Growth Factor antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the Vascular Endothelial Growth Factor antibody.

Storage

Aliquot the Vascular Endothelial Growth Factor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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