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Home >> Antibodies >> VASA Antibody / DDX4

VASA Antibody / DDX4 (R31067)

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  Catalog No Formulation Size Price (USD)  
Image R31067 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of VASA antibody and Lane 1: rat testis; 2: mouse testis; 3: HeLa cell lysate
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Western blot testing of VASA antibody and Lane 1: rat testis; 2: mouse testis; 3: HeLa cell lysate
Western blot testing of VASA antibody and Lane 1: rat testis; 2: mouse testis; 3: (m) ovary tissue lysate
ICC testing of VASA antibody and MCF-7 cell
IHC-P testing of rat testis tissue
IHC-P: VASA antibody testing of rat ovary tissue
IHC-F testing of rat intestine tissue
IHC staining of FFPE human ovarian cancer with VISA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Flow cytometry testing of human PC-3 cells with VISA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VISA antibody.
Flow cytometry testing of human SiHa cells with VISA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VISA antibody.
Immunofluorescent staining of FFPE human U-2 OS cells with VASA antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt Q9NQI0
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Immunohistochemistry (Frozen) : 0.5-1ug/ml
Immunocytochemistry : 2ug/ml
Flow cytometry : 1-3ug/million cells
Immunofluorescence : 5ug/ml
Limitations This VASA antibody is available for research use only.
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Description

DEAD/H BOX 4, also known as DDX4 and VASA contains the 8 conserved domains found in all known DEAD box proteins. The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins. By radiation hybrid analysis, Castrillon et al.(2000) mapped the gene to 5q. By fluorescence in situ hybridization, they refined the localization to 5q11.2-q12. This region is syntenic to the distal end of mouse chromosome 13, where the mouse homolog resides(Abe and Noce, 1997). Using a combination of proteomics, cytology, and functional analysis in C. elegans, Chu et al.(2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the VASA antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids 253-272 (EDEDSIFAHYQTGINFDKYD) were used as the immunogen for this VASA antibody (100% human, mouse and rat homology).

Storage

After reconstitution, the VASA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Related Products

   1.   View all VASA/DDX4 antibodies

   2.   View all DDX antibodies

   3.   View all VASA antibodies

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