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Home >> Antibodies >> VAChT Antibody / Vesicular acetylcholine transporter

VAChT Antibody / Vesicular acetylcholine transporter (R32623)

  Catalog No Formulation Size Price (USD)  
Image R32623 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) human HeLa and 2) mouse HEPA cell lysate with VAChT antibody at 0.5ug/ml. Predicted molecular weight ~57 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt Q16572
Applications Western blot : 0.5-1ug/ml
Limitations This VAChT antibody is available for research use only.
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Description

The Vesicular acetylcholine transporter (VAChT), also known as SLC18A3, is a neurotransmitter transporter which is responsible for loadingacetylcholine (ACh) into secretory organelles in neurons making acetylcholine available for secretion. It is encoded by Solute carrier family 18, member 3 (SLC18A3) gene. This gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.

Application Notes

Optimal dilution of the VAChT antibody should be determined by the researcher.

Immunogen

Amino acids 1-36 (MESAEPAGQARAAATKLSEAVGAALQEPRRQRRLVL-human) were used as the immunogen for the VAChT antibody.

Storage

After reconstitution, the VAChT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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