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Home >> Antibodies >> USP14 Antibody

USP14 Antibody (F54397)

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  Catalog No Formulation Size Price (USD)  
Image F54397-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54397-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of human A2058 cell lysate with USP14 antibody. Predicted molecular weight ~56 kDa.
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Western blot testing of human A2058 cell lysate with USP14 antibody. Predicted molecular weight ~56 kDa.
Western blot testing of human HCT-116 cell lysate with USP14 antibody. Predicted molecular weight ~56 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with USP14 antibody.
IHC testing of FFPE human cancer tissue with USP14 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity SAS precipitation
UniProt P54578
Localization Cytoplasmic
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:25
Limitations This USP14 antibody is available for research use only.
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Description

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Application Notes

The stated application concentrations are suggested starting points. Titration of the USP14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 1-30 from the human protein was used as the immunogen for the USP14 antibody.

Storage

Aliquot the USP14 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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