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Home >> Antibodies >> TUBA1 Antibody / TUBA1A/B/C / Tubulin alpha

TUBA1 Antibody / TUBA1A/B/C / Tubulin alpha [clone 7B12] (RQ6290)

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  Catalog No Formulation Size Price (USD)  
Image RQ6290 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human pancreatic cancer with TUBA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human pancreatic cancer with TUBA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with TUBA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian serous adenocarcinoma with TUBA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with TUBA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with TUBA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain with TUBA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain with TUBA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A431 cells with TUBA1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human Caco-2 cells with TUBA1 antibody (red) and a nuclear counterstain (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) Jurkat, 3) HEK293, 4) K562, 5) rat brain, 6) rat C6 and 7) mouse brain lysate with TUBA1 antibody. Predicted molecular weight ~50 kDa.
Flow cytometry testing of human A431 cells with TUBA1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TUBA1 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name 7B12
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q71U36/P68363/Q9BQE3
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This TUBA1 antibody is available for research use only.
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Description

Tubulin is the major constituent of microtubules. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins.

Application Notes

Optimal dilution of the TUBA1 antibody should be determined by the researcher.

Immunogen

A human recombinant partial protein (amino acids N18-A403) was used as the immunogen for the TUBA1 antibody.

Storage

After reconstitution, the TUBA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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